Mouse AKT2 Antibody Pair - BSA and Azide free (ab241767)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Mouse AKT2 Antibody Pair - BSA and Azide free
See all AKT2 kits -
Assay type
ELISA set -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
The Antibody Pair can be used to quantify AKT2. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab208986), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
-
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse AKT2 Capture Antibody (unconjugated) 1 x 100µg Mouse AKT2 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
-
Function
General protein kinase capable of phosphorylating several known proteins. -
Tissue specificity
Expressed in all human cell types so far analyzed. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. - Information by UniProt
-
Alternative names
- Akt2
- AKT2_HUMAN
- HIHGHH
see all -
Database links
- Entrez Gene: 208 Human
- Entrez Gene: 11652 Mouse
- Entrez Gene: 25233 Rat
- Omim: 164731 Human
- SwissProt: P31751 Human
- SwissProt: Q60823 Mouse
- SwissProt: P47197 Rat
- Unigene: 631535 Human
see all
Images
-
Sandwich ELISA - Mouse AKT2 Antibody Pair - BSA and Azide free (ab241767)To learn more about the advantages of recombinant antibodies see here.
