Xanthine Oxidase Activity Assay Kit (ab102522)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture extracts, Milk, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 10 mU/ml
Overview
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Product name
Xanthine Oxidase Activity Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
> 10 mU/ml -
Range
10 mU/ml - 1000 mU/ml -
Assay time
0h 40m -
Product overview
Xanthine Oxidase Activity Assay Kit ab102522 is an easy and sensitive assay to determine xanthine oxidase activity in variety of samples.
In the xanthine oxidase assay protocol, xanthine oxidase oxidizes xanthine to hydrogen peroxide (H2O2) which reacts stoichiometrically with a probe to generate color (at OD = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured.
The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.
Xanthine oxidase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader immediately and after 10-20 min -
Notes
Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests H2O2 Standard (0.88 M) Yellow 1 x 0.1ml OxiRed™ Probe (in DMSO) Red 1 x 200µl XO Assay Buffer WM 1 x 25ml XO Enzyme Mix (Lyophilized) Green 1 vial XO Positive Control Blue 1 x 8µl XO Substrate Mix (Lyophilized) Purple 1 vial -
Research areas
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Relevance
Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice. -
Cellular localization
Cytoplasm. Peroxisome. Secreted. -
Alternative names
- Xanthine dehydrogenase/oxidase
- Xanthine oxidoreductase
- XO
- XOR
Images
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Functional Studies - Xanthine Oxidase Assay Kit (ab102522)
Representative Standard Curve using ab102522.
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Functional Studies - Xanthine Oxidase Assay Kit (ab102522)Xanthine Oxidase Positive Control.