Mouse KIM-1 ELISA Kit (TIM1) (ab213477)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.6 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse KIM-1 ELISA Kit (TIM1)
See all TIM 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 2.2% Inter-assay Sample n Mean SD CV% Overall 3 3.2% -
Sample type
Cell culture supernatant, Urine, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.6 pg/ml -
Range
6.25 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 102 95% - 112% Serum 103 88% - 128% Tissue Extracts 96 90% - 100% Hep Plasma 106 98% - 112% EDTA Plasma 107 96% - 119% Cit plasma 82 74% - 89% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse KIM-1 ELISA Kit (TIM1) (ab213477) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of KIM-1 (TIM1) protein in serum, tissue extracts, urine, cit plasma, edta plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse KIM-1 (TIM1) with 1.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
KIM-1, also known as Kidney Injury Molecule 1 and Hepatitis A virus cellular receptor 1 homolog (HAVcr-1), or T-cell immunoglobulin and mucin domain 1 (TIM-1) is a 283 amino acid protein that contains an N-terminal immunoglobulin-like domain and is encoded by the HAVCR1 gene. KIM-1 is a single-pass type 1 membrane protein has been identified as a receptor for hepatitis A virus. The TIM gene family participates in host immune response. Urinary KIM-1 levels are elevated in nephropathy and is thought to be a biomarker for renal damage.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse KIM1 Capture Antibody 1 x 600µl 10X Mouse KIM1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse KIM1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 75BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. -
Tissue specificity
Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. -
Sequence similarities
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD365
- HAVCR
- HAVCR 1
see all -
Database links
- Entrez Gene: 171283 Mouse
- SwissProt: Q5QNS5 Mouse
Images
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Other - Mouse KIM-1 ELISA Kit (TIM1) (ab213477)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse KIM-1 standard curve comparison data.Standard curve comparison between mouse KIM-1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.
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Example of mouse KIM-1 standard curve in Sample Diluent NS, when applicable.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse KIM-1 standard curve in Sample Diluent 75BP, when applicable.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse KIM-1 standard curve in 1X Cell Extraction Buffer PTR, when applicable.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native KIM-1 in mouse serum, and plasma samples.The concentrations of KIM-1 were measured in duplicates, interpolated from the KIM-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 90%, plasma (citrate) 90%, plasma (heparin) 90% and plasma (EDTA) 90%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean KIM-1 concentration was determined to be 170 pg/mL in serum, 125 pg/mL in plasma (citrate), 181 pg/mL in plasma (heparin), and160 pg/mL in plasma (EDTA).
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Interpolated concentrations of native mouse KIM-1 in mouse kidney supernatant, urine, and extract based on a 25%, 50%, and 50 µg/mL extract load, respectively.The concentrations of KIM-1 were measured in duplicate and interpolated from the KIM-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean KIM-1 concentration was determined to be 1,112 pg/mL in neat kidney supernatant, 505 pg/mL in neat urine, and 219 pg/mL in kidney extract, based on a 50 μg/mL extract load.
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Sandwich ELISA - Mouse KIM-1 ELISA Kit (TIM1) (ab213477)To learn more about the advantages of recombinant antibodies see here.
