Anti-BLNK antibody (ab2230)
Key features and details
- Goat polyclonal to BLNK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BLNK antibody
See all BLNK primary antibodies -
Description
Goat polyclonal to BLNK -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide: KDSTRLKYAVKVS, corresponding to C terminal amino acids 444-456 of Human BLNK.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BLNK antibody (ab2230)Anti-BLNK antibody (ab2230) at 4 µg/ml + Cell lysate prepared from human Daudi cells at 35 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Ab2230 staining (4µ g/ml) of Daudi lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence Primary incubated for 1 hour. -
Western blot - Anti-BLNK antibody (ab2230)All lanes : Anti-BLNK antibody (ab2230) at 1 µg/ml
Lane 1 : Cell lysate prepared from HEK293 mock transfected cells
Lane 2 : Cell lysate prepared from HEK293 overexpressing BLNK
Predicted band size: 51 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
