Anti-T54 antibody (ab224209)
Key features and details
- Rabbit polyclonal to T54
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-T54 antibody -
Description
Rabbit polyclonal to T54 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human T54 aa 50-200.
Database link: Q92917 -
Positive control
- WB: RT4, U-251 MG sp, A431, NIH/3T3, NBT-II and PC-12 cell lysates. IHC-P: Human kidney, colon, pancreas and cerebral cortex tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T54 antibody (ab224209)Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling T54 with ab224209 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-T54 antibody (ab224209)All lanes : Anti-T54 antibody (ab224209) at 1/250 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 52 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-T54 antibody (ab224209)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for T54 (green) using ab224209 at 4 µg/ml in ICC/IF.
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Western blot - Anti-T54 antibody (ab224209)All lanes : Anti-T54 antibody (ab224209) at 1/250 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 52 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T54 antibody (ab224209)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling T54 with ab224209 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T54 antibody (ab224209)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling T54 with ab224209 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T54 antibody (ab224209)Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling T54 with ab224209 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T54 antibody (ab224209)Paraffin-embedded human cerebral cortex tissue stained for T54 using ab224209 at 1/20 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T54 antibody (ab224209)Paraffin-embedded human kidney tissue stained for T54 using ab224209 at 1/20 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T54 antibody (ab224209)Paraffin-embedded human colon tissue stained for T54 using ab224209 at 1/20 dilution in immunohistochemical analysis.
