Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PHYHD1 antibody [EPR13313(B)] (ab184174)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13313(B)] to PHYHD1
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-PHYHD1 antibody [EPR13313(B)]
    See all PHYHD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13313(B)] to PHYHD1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver andHuman fetal kidney lysates. HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13313(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PHYHD1 antibody [EPR13313(B)] (ab184174)
    Western blot - Anti-PHYHD1 antibody [EPR13313(B)] (ab184174)
    All lanes : Anti-PHYHD1 antibody [EPR13313(B)] (ab184174) at 1/10000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa



    5% NFDM/TBST blocking/diluting buffer

  • Flow Cytometry - Anti-PHYHD1 antibody [EPR13313(B)] (ab184174)
    Flow Cytometry - Anti-PHYHD1 antibody [EPR13313(B)] (ab184174)

    Flow ctometric analysis of HepG2 cells fixed in 2% paraformaldehyde labeling PHYHD1 with ab184174 at 1/400 dilution (red). Goat andti rabbit IgG (FITC) was used at 1/150 dilution as secondary antibody. Rabbit monoclonal IgG was used as an isotype control (green).

  • Immunocytochemistry/ Immunofluorescence - Anti-PHYHD1 antibody [EPR13313(B)] (ab184174)
    Immunocytochemistry/ Immunofluorescence - Anti-PHYHD1 antibody [EPR13313(B)] (ab184174)

    Immunofluorescent analysis of HepG2 cells fixed in 4% paraformaldehyde labeling PHYHD1 with ab184174 at 1/500 dilution. Goat anti-rabbit IgG(Alexa Fluor® 555) was used at 1/200 dilution for secondary antibody and the cells were counterstained with DAPI.

  • Anti-PHYHD1 antibody [EPR13313(B)] (ab184174)
    Anti-PHYHD1 antibody [EPR13313(B)] (ab184174)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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