20 HETE / beta glucuronidase ELISA Kit (ab175818)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 0.01 ng/ml - 1000 ng/ml
- Sample type: Cell culture media, Cell Lysate, Plasma, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
20 HETE / beta glucuronidase ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 12 = 0.3% Inter-assay Sample n Mean SD CV% Overall 12 = 0.78% -
Sample type
Urine, Serum, Plasma, Tissue, Cell Lysate, Cell culture media -
Assay type
Competitive -
Sensitivity
= 10 pg/ml -
Range
0.01 ng/ml - 1000 ng/ml -
Assay duration
Multiple steps standard assay -
Product overview
Abcam’s 20 HETE / beta glucuronidase competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the determination of free and glucuronidated 20 HETE (also known as 20-OH-AA) levels in biological samples. The specificity of the 20 HETE ELISA was investigated using authentic 20 HETE and a panel of fatty acids which, based on their structure, might be anticipated to compete with 20 HETE for binding to antibodies for 20 HETE. Anti-20 HETE did not cross-react with 14,15- and 11,12-DHETs, PGE2 and showed almost no cross-reactivity even with structurally extremely similar arachidonic acid (AA), linoleic acid and linolenic acid as shown in the competitive ELISA analysis. Considering the only difference between 20 HETE and AA is an oxygen molecule, the specificity of this kit is a surprise.
Human essential and salt-sensitive hypertensions were related to differential AA metabolism by cytochrome P450 (CYP) 4A which has AA-ω-hydroxylase (20 HETE synthesis) activity. Increased circulating insulin inhibits 20 HETE synthesis in obese hypertensive subjects.
Recently, CYP4F2 genetic variants, which increased urinary 20 HETE secretion, were found to be correlated with the risk for hypertension in a Chinese population.
This kit can be used for the determination of free 20 HETE and glucuronidated 20 HETE in serum, plasma, cells and tissues following proper isolation and purification.
This competitive ELISA kit is for determination of free and glucuronidated 20-HETE levels in cell extracts and cell media. The kit is similar to our 20-HETE ELISA Kit (ab175817) but also contains the beta-glucuronidase enzyme. Each 96-well plate is sufficient for triplicate analyses of up to 12 free and 12 glucuronidated samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 1000X 20-HETE HRP Conjugates 1 x 12µl 20-HETE Standard 1 x 2µl Beta-Glucuronidase enzyme 1 x 8mg HRP Buffer 1 x 15ml Pre-Coated 96-Well Microplate 1 unit 10X Sample Dilution Buffer 1 x 25ml TMB Substrate 1 x 24ml 10X Wash Buffer Solution 1 x 25ml -
Research areas
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Alternative names
- cytochrome P450,20-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid
Images
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Typical Standard Curve
Example of standard curve obtained using ab175818.
