NADPH Assay Kit (Colorimetric) (ab186031)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell culture supernatant, Cell Lysate, Tissue
- Sensitivity: 3 µM
Overview
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Product name
NADPH Assay Kit (Colorimetric)
See all NADPH kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Tissue, Cell Lysate -
Assay type
Quantitative -
Sensitivity
> 3 µM -
Range
3.13 µM - 100 µM -
Assay time
2h 00m -
Product overview
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+. NAD forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. The traditional NAD/NADH and NADP/NADPH assays are based on monitoring the changes in NADH or NADPH absorption at 340 nm. The short UV wavelength of NAD/NADH and NADP/NADPH assays makes the traditional methods suffer low sensitivity and high interference.
Abcam’s Colorimetric NADPH Assay Kit (ab186031) provides a convenient method for detecting NADPH. The NADPH probe is a chromogenic sensor that has its maximum absorbance at 460 nm upon NADPH reduction. The absorbance maximum increases to ~ 635 nm if the enhancer is added to the assay system. The absorption of the NADPH probe is directly proportional to the concentration of NADPH in the solution. The Colorimetric NADPH Assay Kit provides a sensitive assay to detect as little as 3 µM NADPH in a 100 µL assay volume.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 400 tests Assay Buffer 1 x 16ml Lysis Buffer 1 x 10ml NADPH Probe 1 x 4ml NADPH Standard 1 x 167µg -
Research areas
Images
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NADPH standard curve
NADPH dose response was measured with the Colorimetric NADPH Assay Kit in a 96-well white/clear bottom plate using a microplate reader. As low as 3 µM of NAPDH can be detected with 30min incubation (n=3) with absorbance measurement at 460nm.
The absorbance in blank wells (with the PBS buffer only) is used as a control, and is subtracted from the values for those wells with the NADPH reactions.
