Cellular ROS/RNS Assay Kit (ab139473)
Key features and details
- Assay type: Cell-based
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cellular ROS/RNS Assay Kit
See all Oxidative Stress kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Cellular ROS/RNS Assay Kit (ab139473) is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy. The kit includes three fluorescent dye reagents: NO Detection Reagent (Red), Oxidative Stress Detection Reagent (Green) for total ROS detection, and Superoxide Detection Reagent (Orange). It also includes positive controls, pyocyanin and L-arginine, which are common inducers of ROS and NO production, respectively, as well as negative controls, N-acetyl-L-cysteine and c-PTIO (common scavengers of ROS and NO, respectively). Through the combination of the three detection probes with a set of specific inhibitors and activators, this kit enables discrimination among superoxide, nitric oxide and peroxynitrite.
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Notes
Related products
Review the oxidative stress marker and assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Please refer to protocols. -
Components 200 tests 10 X Wash Buffer 1 x 15ml NO Detection Reagent (Red) 1 x 60µl NO Inducer (L-Arginine) 1 x 100µl NO Scavenger (c-PTIO) 1 x 400nmole Oxidative Stress Detection Reagent (Green) 1 x 300nmole ROS Inducer (Pyocyanin 1 µmole) 1 vial ROS Inhibitor (N-acetyl-L-cysteine) 2 x 10mg Superoxide Detection Reagent (Orange) 1 x 300nmole -
Research areas
