Recombinant dog PDE3A protein (Active) (ab271677)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 70% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant dog PDE3A protein (Active)
See all PDE3A proteins and peptides -
Biological activity
Assay conditions:10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 μM cAMP, 0.02% TritonX, and serial dilutions of PDE3A at 37ºC for 20 mins. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Absorbance was measured at 620 nm. -
Purity
>= 70 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Dog -
Predicted molecular weight
75 kDa -
Amino acids
343 to 998 -
Tags
DDDDK tag C-Terminus -
Additional sequence information
Genbank: XP_005637137.
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Preparation and Storage
- 5''-cyclic phosphodiesterase A
- cAMP phosphodiesterase myocardial cGMP inhibited
- CGI PDE
Images
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SDS-PAGE - Recombinant dog PDE3A protein (Active) (ab271677)
SDS-PAGE analysis of 2 µg ab271677.
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Functional Studies - Recombinant dog PDE3A protein (Active) (ab271677)Specific activity of ab271677.