Recombinant mouse PDE2A protein (Active) (ab271675)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 89% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant mouse PDE2A protein (Active)
See all PDE2A proteins and peptides -
Biological activity
Specific Activity: ≥20,000 pmole/min/µg.One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE2A at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
Purity
>= 89 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Predicted molecular weight
130 kDa including tags -
Amino acids
2 to 939 -
Tags
GST tag N-Terminus
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Preparation and Storage
- 5''-cyclic phosphodiesterase
- cGMP dependent 3'5' cyclic phosphodiesterase
- cGMP stimulated phosphodiesterase 1
Images
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Functional Studies - Recombinant mouse PDE2A protein (Active) (ab271675)
Specific activity of ab271675 was ≥20,000 pmole/min/µg.
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SDS-PAGE - Recombinant mouse PDE2A protein (Active) (ab271675)SDS-PAGE analysis of 1.9 µg ab271675.