Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TORC1 antibody [EPR3382] - BSA and Azide free (ab247563)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3382] to TORC1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-TORC1 antibody [EPR3382] - BSA and Azide free
    See all TORC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3382] to TORC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247563 is the carrier-free version of ab92477.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-TORC1 antibody [EPR3382] - BSA and Azide free (ab247563)
    Flow Cytometry - Anti-TORC1 antibody [EPR3382] - BSA and Azide free (ab247563)
    This data was developed using ab92477, the same antibody clone in a different buffer formulation.Overlay histogram showing SHSY-5Y cells stained with ab92477 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92477, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Western blot - Anti-TORC1 antibody [EPR3382] - BSA and Azide free (ab247563)
    Western blot - Anti-TORC1 antibody [EPR3382] - BSA and Azide free (ab247563)
    All lanes : Anti-TORC1 antibody [EPR3382] (ab92477) at 1/1000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : C6 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution

    Predicted band size: 67 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab92477, the same antibody clone in a different buffer formulation.

     

  • Anti-TORC1 antibody [EPR3382] - BSA and Azide free (ab247563)
    Anti-TORC1 antibody [EPR3382] - BSA and Azide free (ab247563)

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