Anti-EN2 antibody (ab227167)
Key features and details
- Rabbit polyclonal to EN2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EN2 antibody
See all EN2 primary antibodies -
Description
Rabbit polyclonal to EN2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human EN2.
Database link: P19622 -
Positive control
- WB: MCF7 whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EN2 antibody (ab227167)Anti-EN2 antibody (ab227167) at 1/10000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 34 kDa12% SDS-PAGE gel.
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Immunoprecipitation - Anti-EN2 antibody (ab227167)EN2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2 µg of ab227167. Western blot was performed from the immunoprecipitate using ab227167 at 1/1000 dilution. Anti-Rabbit IgG was used as secondary antibody.
Lane 1: HeLa whole cell lysate 40 µg (Input).
Lane 2: 2 µg preimmune rabbit IgG instead of ab227167 in HeLa whole cell lysate.
Lane 3: ab227167 IP in HeLa whole cell lysate.
12% SDS-PAGE.
