Anti-Dnmt3b antibody (ab122932)
Key features and details
- Rabbit polyclonal to Dnmt3b
- Suitable for: IHC-Fr, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Dnmt3b antibody
See all Dnmt3b primary antibodies -
Description
Rabbit polyclonal to Dnmt3b -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to amino acids 1-181 of Mouse Dnmt3b (AF068626)
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Positive control
- Solubilized mouse embryos at the stages of E7.0-E16.5; transiently transfected 293T cells; E4.5, 5.5 and 7.0 mouse embryo sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), Ammonium sulphate
trace of ammonium sulphate only -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Dnmt3b antibody (ab122932)All lanes : Anti-Dnmt3b antibody (ab122932) at 1/3000 dilution
Lane 1 : wild-type mouse embryonic stem cell lysate with 5% skim milk
Lane 2 : Dnmt3b/3a knockout mouse embryonic stem cell lysate with 5% skim milk
Predicted band size: 96 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3b antibody (ab122932)Immunocytochemistry/ Immunofluorescence analysis of wild-type (left) and Dnmt3b/3a knockout (right) mouse embryonic stem cells using ab122932 at 1/5000 dilution. DNA was stained with DAPI (bottom). Cell were fixed with 4% PFA/PBS for 30 min at 4℃.
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Western blot - Anti-Dnmt3b antibody (ab122932)All lanes : Anti-Dnmt3b antibody (ab122932) at 1/1000 dilution
Lane 1 : stages E7.0 solubilized mouse embryo at 10 µg
Lane 2 : stages E8.5 solubilized mouse embryo at 10 µg
Lane 3 : stages E9.5 solubilized mouse embryo at 10 µg
Lane 4 : stages E10.5 solubilized mouse embryo at 10 µg
Lane 5 : stages E12.5 solubilized mouse embryo at 10 µg
Lane 6 : stages E14.5 solubilized mouse embryo at 10 µg
Lane 7 : stages E16.5 solubilized mouse embryo at 10 µg
Lane 8 : 293T cells transiently transfected with 3b1 isoform of Dnmt3b
Lane 9 : 293T cells transiently transfected with 3b2 isoform of Dnmt3b
Lane 10 : 293T cells transiently transfected with 3b3 isoform of Dnmt3b
Predicted band size: 96 kDa
The embryos were solubilized by sonication or homogenization in the presence of 0.1% SDS. -
Immunohistochemistry (Frozen sections) - Anti-Dnmt3b antibody (ab122932)Expression of Dnmt3b in mouse embyos was examined by immuno-fluorescence staining using 1/1000 ab122932.
Mouse embryos at E4.5, 5.5 and 7.0 were fixed in cold acetone and stained with ab122932 or DAPI (blue). The antibody was detected with anti-rabbit IgG conjugated with ALEXA568 (red). The ICM (the inner cell mass) and trophectoderm (T) are indicated by arrows and arrowheads, respectively. The epiblast (E), and the embryonic ectoderm (EE) and extraembryonic region (EX) are indicated by square brackets. Dnmt3b existed at E4.5-7.0, in ICM at E4.5, the epiblast at E5.5, and the embryonic ectoderm at E7.0.
