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Epigenetics and Nuclear Signaling DNA methylation DNMTs

DNMT3A Assay Kit (ab113470)

DNMT3A Assay Kit (ab113470)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Assay type: Quantitative
  • Platform: Microplate reader
  • Assay time: 3 hr

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Overview

  • Product name

    DNMT3A Assay Kit
  • Assay type

    Quantitative
  • Assay time

    3h 00m
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Product overview

    DNMT3A (DNA (cytosine-5)-methyltransferase 3A) belongs to the DNMT family, and it is required for genome wide de novo methylation and it is essential for the establishment of DNA methylation patterns during development.

     DNMT3A Assay Kit (ab113470) allows the user to quickly and consistently measure the amount of DNMT3A. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT3A assay experiment. ab113470 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.

    Not sure if this is the right product for you? Check out our EpiSeeker DNMT Product Guide for help.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 48 tests 96 tests
    10X Wash Buffer 1 x 12ml 1 x 25ml
    8-Well Assay Strips (with Frame) 6 units 12 units
    Affinity Antibody, 100 µg/mL 1 x 8µl 1 x 16µl
    Assay Buffer 1 x 5ml 1 x 10ml
    Blocking Buffer 1 x 10ml 1 x 20ml
    Detection Antibody, 200 µg/mL 1 x 10µl 1 x 20µl
    Developing Solution 1 x 6ml 1 x 12ml
    DNMT3A Standard, 20 µg/mL 1 x 16µl 1 x 30µl
    Stop Solution 1 x 6ml 1 x 11ml
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • DNMTs
    • Kits/ Lysates/ Other
    • Kits
    • Epigenetic kits
    • DNA modification and methylation

Images

  • Functional Studies - DNMT3A Assay Kit (ab113470)
    Functional Studies - DNMT3A Assay Kit (ab113470)

    Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (ab113474) and DNMT3A activity was measured using ab113470. DNMT1 and DNMT3B activities were measured using ab113469 DNMT1 Assay Kit and ab113471 DNMT3B Assay Kit.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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