Anti-Dnmt3b antibody (ab2851)
Key features and details
- Rabbit polyclonal to Dnmt3b
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Dnmt3b antibody
See all Dnmt3b primary antibodies -
Description
Rabbit polyclonal to Dnmt3b -
Host species
Rabbit -
Specificity
This antibody detects DNA methyltransferase 3b (Dnmt3b) from human and mouse tissues and cells as well as recombinant human Dnmt3b. This antibody detects, to a lesser extent, full-length human recombinant Dnmt3a. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Mouse Dnmt3b aa 1-14.
Sequence:MKGDSRHLNEEEGA
(Peptide available asab4922)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3b antibody (ab2851)
ab2851 labellling Dnmt3b in the nucleus of Mouse brain tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissue sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Western blot - Anti-Dnmt3b antibody (ab2851)All lanes : Anti-Dnmt3b antibody (ab2851) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : NIH-3T3 cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 97.5 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3b antibody (ab2851)ab2851 labellling Dnmt3b in the nucleus of Human testis tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissue sections were incubated with primary antibody (1:500 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3b antibody (ab2851)ab2851 labellling Dnmt3b in the nucleus and cytoplasm of Human breast carcinoma (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissue sections were incubated with primary antibody (1:500 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Western blot - Anti-Dnmt3b antibody (ab2851)All lanes : Anti-Dnmt3b antibody (ab2851) at 1 µg/ml
Lane 1 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97.5 kDa
Observed band size: 97.5 kDa
Additional bands at: 32 kDa, 46 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
