Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Dnmt3b antibody [EPR3523] (ab79822)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3523] to Dnmt3b
Suitable for: WB
Reacts with: Mouse, Human

Product name

Anti-Dnmt3b antibody [EPR3523]
See all Dnmt3b primary antibodies
Description

Rabbit monoclonal [EPR3523] to Dnmt3b
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human Dnmt3b aa 50-150. The exact sequence is proprietary.
Positive control
- A549, HeLa and A431 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3523
Isotype

IgG
Research areas

Western blot - Anti-Dnmt3b antibody [EPR3523] (ab79822) This image is courtesy of an anonymous abreview.

All lanes : Anti-Dnmt3b antibody [EPR3523] (ab79822) at 1/1000 dilution

Lane 1 : Flag-tagged Dnmt3b
Lane 2 : Dnmt3b knockout mouse embryonic stem cell extract
Lanes 3-4 : Wild type mouse embryonic stem cell extract

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Donkey anti-rabbit IgG conjugated to HRP (NA934) at 1/1000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 96 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Exposure time: 5 minutes

Blocking: 5% Milk
See Abreview
Western blot - Anti-Dnmt3b antibody [EPR3523] (ab79822)

All lanes : Anti-Dnmt3b antibody [EPR3523] (ab79822) at 1/5000 dilution

Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

Predicted band size: 96 kDa
Observed band size: 96 kDa
Anti-Dnmt3b antibody [EPR3523] (ab79822)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com