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Epigenetics and Nuclear Signaling DNA methylation DNMTs

Anti-Dnmt1 antibody [2C1] (ab252825)

Price and availability

307 566 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Dnmt1 antibody [2C1] (ab252825)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [2C1] to Dnmt1
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Dnmt1 antibody [2C1]
    See all Dnmt1 primary antibodies
  • Description

    Rat monoclonal [2C1] to Dnmt1
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human Dnmt1 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P26358

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: NCI-H1299, HEK-293, HeLa, Jurkat and HepG2 whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    2C1
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • DNMTs

Images

  • Western blot - Anti-Dnmt1 antibody [2C1] (ab252825)
    Western blot - Anti-Dnmt1 antibody [2C1] (ab252825)
    All lanes : Anti-Dnmt1 antibody [2C1] (ab252825) at 0.353 µg/ml

    Lane 1 : NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 5 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/100000 dilution

    Predicted band size: 183 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Dnmt1 antibody [2C1] (ab252825)
    Anti-Dnmt1 antibody [2C1] (ab252825)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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