Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MGMT antibody [MT3.1] - BSA and Azide free (ab255966)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [MT3.1] to MGMT - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MGMT antibody [MT3.1] - BSA and Azide free
    See all MGMT primary antibodies

  • Description

    Mouse monoclonal [MT3.1] to MGMT - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human prostate carcinoma and lung carcinoma tissue. WB: MCF7, HeLa, Jurkat and HCT116 whole cell lysate. Wild type HAP1 whole cell lysate.

  • General notes

    ab255966 is the carrier-free version of ab39253. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255966 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MGMT antibody [MT3.1] - BSA and Azide free (ab255966)
    Western blot - Anti-MGMT antibody [MT3.1] - BSA and Azide free (ab255966)
    All lanes : Anti-MGMT antibody [MT3.1] (ab39253) at 1/500 dilution

    Lane 1 : Wild type HAP1 whole cell lysate
    Lane 2 : MGMT knockout HAP1 whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell)
    Lane 4 : Jurkat (human T cell leukemia T lymphocyte)

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 22 kDa



    This data was produced using the same clone but in a different formulation, ab39253.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [MT3.1] - BSA and Azide free (ab255966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [MT3.1] - BSA and Azide free (ab255966)

    This data was produced using the same clone but in a different formulation, ab39253.

    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue labeling MGMT with ab39253 at 1/200 followed by a ready to use secondary. Positive staining on human prostate carcinoma. The section was incubated with ab39253 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use secondary. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-MGMT antibody [MT3.1] - BSA and Azide free (ab255966)
    Western blot - Anti-MGMT antibody [MT3.1] - BSA and Azide free (ab255966)
    All lanes : Anti-MGMT antibody [MT3.1] (ab39253) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 4 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 22 kDa


    Exposure time: 92 seconds


    This data was produced using the same clone but in a different formulation, ab39253.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [MT3.1] - BSA and Azide free (ab255966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGMT antibody [MT3.1] - BSA and Azide free (ab255966)

    This data was produced using the same clone but in a different formulation, ab39253.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling MGMT with ab39253 at 1/200 followed by a ready to use secondary. Positive staining on human lung carcinoma. The section was incubated with ab39253 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use secondary. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-MGMT antibody [MT3.1] - BSA and Azide free (ab255966)
    Anti-MGMT antibody [MT3.1] - BSA and Azide free (ab255966)

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