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Anti-Dnmt1 antibody [60B1220.1] (ab13537)

Key features and details

  • Mouse monoclonal [60B1220.1] to Dnmt1
  • Suitable for: Flow Cyt, IHC-Fr, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Dnmt1 antibody [60B1220.1]
    See all Dnmt1 primary antibodies

  • Description

    Mouse monoclonal [60B1220.1] to Dnmt1

  • Host species

    Mouse

  • Specificity

    This antibody detects a ~180 kDa protein, corresponding to the apparent molecular mass of Dnmt1 on SDS-PAGE immunoblots in samples of human and mouse origin. Immunogen itself has been shown to be toxic.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide:

    EKDDREDKENAFKR

    , corresponding to amino acids 637-650 of Human Dnmt1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: H1299 cell lysate. IHC-Fr: Human colon carcinoma tissue. Mouse medullar kidney tissue. Flow Cyt: HeLa cells.

  • General notes

    For maximum product recovery, centrifuge the product vial before removing cap. Shipped at on gel packs.

    DNMT1 high levels are toxic, as a result it may be tough to find sometimes. Signal amplification might be needed.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    This antibody is affinity purified.

  • Clonality

    Monoclonal

  • Clone number

    60B1220.1

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-Dnmt1 antibody [60B1220.1] (ab13537)
    Western blot - Anti-Dnmt1 antibody [60B1220.1] (ab13537)
    Anti-Dnmt1 antibody [60B1220.1] (ab13537) at 1/1000 dilution + H1299 (Human non-small cell lung carcinoma cell line) lysate

    Predicted band size: 183 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Dnmt1 antibody [60B1220.1] (ab13537)
    Immunohistochemistry (Frozen sections) - Anti-Dnmt1 antibody [60B1220.1] (ab13537)

    ab13537 at 1/10000 dilution (12 hours at 4degC) staining Dnmt1 in human colon carcinoma tissue section by Immunohistochemistry (Formalin-fixed tissue sections). A biotin Goat Anti-Mouse secondary was used at 1/2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200µl for 2 mins at RT.

  • Flow Cytometry - Anti-Dnmt1 antibody [60B1220.1] (ab13537)
    Flow Cytometry - Anti-Dnmt1 antibody [60B1220.1] (ab13537)
    Overlay histogram showing HeLa cells stained with ab13537 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13537, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemistry (Frozen sections) - Anti-Dnmt1 antibody [60B1220.1] (ab13537)
    Immunohistochemistry (Frozen sections) - Anti-Dnmt1 antibody [60B1220.1] (ab13537)

    Mouse medullar kidney tissue stained for Dnmt1 using ab13537 at 1/1000 dilution in immunohistochemical analysis. DAB staining. Counterstained with hematoxylin.

  • Western blot - Anti-Dnmt1 antibody [60B1220.1] (ab13537)
    Western blot - Anti-Dnmt1 antibody [60B1220.1] (ab13537) This image is courtesy of an anonymous Abreview
    All lanes : Anti-Dnmt1 antibody [60B1220.1] (ab13537) at 1 µg/ml

    Lane 1 : HCT116 whole cell lysate
    Lane 2 : HCT116 DNMT1 KO whole cell lysate
    Lane 3 : HCT116 DNMT3b KO whole cell lysate

    Secondary
    All lanes : HRP conjugated Sheep anti-mouse IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 183 kDa


    Exposure time: 1 minute

    See Abreview

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