Feline IL-5 ELISA Kit (ab273235)
Key features and details
- Sensitivity: 4.5 pg/ml
- Range: 4.096 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cat
Overview
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Product name
Feline IL-5 ELISA Kit
See all IL-5 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.5 pg/ml -
Range
4.096 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 123.8 114% - 134% Plasma 115.5 107% - 121% Cell culture media 139.6 125% - 148% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cat -
Product overview
Feline IL-5 ELISA Kit (ab273235) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline IL-5 in serum (feline IL-5 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Feline IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Feline IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Feline IL-5 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Feline IL-5 Antibody 2 vials Feline IL-5 Standard (Lyophilized) 2 vials 200X HRP-Streptavidin Concentrate 1 x 200µl Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. -
Sequence similarities
Belongs to the IL-5 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- B-cell differentiation factor I
- Colony stimulating factor
- EDF
see all
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.