Cellular Superoxide Detection Assay Kit (ab139477)
Key features and details
- Assay type: Cell-based
- Platform: Flow cytometer, Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cellular Superoxide Detection Assay Kit -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Product overview
Cellular Superoxide Detection Assay Kit (ab139477) is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry. A major component of the kit, Superoxide Detection Reagent (Orange), is a cell-permeable probe that reacts specifically with superoxide, generating an orange fluorescent product. The kit is not designed to detect reactive peroxide, hydroxyl, peroxynitrite, chlorine or bromine species, as the fluorescent probe included is relatively insensitive to these analytes. Upon staining, the fluorescent product generated can be visualized using a wide-field fluorescence microscope equipped with standard orange (e.g. 550/620 nm) or red (e.g. 650/670 nm) fluorescent cubes, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser.
The Cellular Superoxide Detection Assay kit contains sufficient reagents for at least 200 microscopy assays or 50 flow cytometry assays using live cells (adherent or in suspension).
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Notes
Related products
Review the oxidative stress marker and assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
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Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Please refer to protocols. -
Components 1 kit ROS Inducer (Pyocyanin 1 µmole) 1 vial ROS Inhibitor (N-acetyl-L-cysteine) 2 x 10mg Superoxide Detection Reagent (Orange) 1 x 300nmole Wash Buffer Salts 1 pack -
Research areas
Images
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Flow cytometric analysis of Jurkat cells
Cellular Superoxide Detection Assay Kit (ab139477) Jurkat cells were induced with 100 µM pyocyanin (general ROS inducer, panel A), or 200 µM antimycin A (superoxide inducer, panel B), stained with Superoxide Detection Reagent (Orange) and analyzed using flow cytometry. Untreated cells (tinted profile) were used as a control. Cell debris were ungated. The numbers within the inserts reflect the mean orange fluorescence of the cells treated and control samples.