Anti-EN2 antibody (ab45867)
Key features and details
- Goat polyclonal to EN2
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-EN2 antibody
See all EN2 primary antibodies -
Description
Goat polyclonal to EN2 -
Host species
Goat -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Dog
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Immunogen
Synthetic peptide:
C-NHSTTAKEGKSDSE
, corresponding to C terminal amino acids 320-333 of Human EN2 -
Positive control
- WB: Mouse brain lysate; ICC: HEK293 and U2OS cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EN2 antibody (ab45867)Anti-EN2 antibody (ab45867) at 1 µg/ml + Mouse brain lysate at 35 µg
Predicted band size: 34 kDa
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Immunocytochemistry - Anti-EN2 antibody (ab45867)Immunocytochemistry analysis of U2OS cells labelling EN2 with ab45867 at 10 μg/mL. Cells fixed with paraformaldehyde, and permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor 488 secondary antibody (2 μg/mL), showing nuclear staining (green). Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL).
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Immunocytochemistry - Anti-EN2 antibody (ab45867)Immunocytochemistry analysis of HEK293 cells labelling EN2 with ab45867 at 10 μg/mL. Cells fixed with paraformaldehyde, and permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor 488 secondary antibody (2 μg/mL), showing nuclear staining (green). Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL).
