Human BNP ELISA Kit (ab193694)
Key features and details
- Sensitivity: 14 pg/ml
- Range: 14 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human BNP ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
14 pg/ml -
Range
14 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 78.12 68% - 83% Plasma 80.96 67% - 107% Cell culture media 94.31 83% - 105% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s BNP Human ELISA Kit (ab193694) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BNP in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human BNP coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures BNP present in the samples. The wells are washed and biotinylated anti-Human BNP antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of BNP bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Human BNP Detection Antibody (lyophilized) 2 vials Human BNP Standards (lyophilized) 2 vials Pre-coated Human BNP Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Function
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Tissue specificity
Brain and also in atria, but at much lower levels than ANP. -
Sequence similarities
Belongs to the natriuretic peptide family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANFB_HUMAN
- BNP(1-32)
- BNP(5-29)
see all -
Database links
- Entrez Gene: 4879 Human
- Omim: 600295 Human
- SwissProt: P16860 Human
- Unigene: 219140 Human
Images
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Standard Curve.
Standard curve using ab193694 BNP Human ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.