Cellular Antioxidant Assay Kit (ab242300)
Key features and details
- Detection method: Fluorescent
- Sample type: Adherent cells
Overview
-
Product name
Cellular Antioxidant Assay Kit
See all Oxidative Stress kits -
Detection method
Fluorescent -
Sample type
Adherent cells -
Product overview
Cellular Antioxidant Assay Kit (ab242300) is a cell-based assay for measuring antioxidant activity within adherent cells.
Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.
Fluorescence is measured over time in a standard microplate fluorometer. This fluorescence correlates to the Quercetin’s ability to quench free radicals. Test antioxidant values can be compared to Quercetin to determine antioxidant activity within the cell.
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 192 tests 96-Well Cell Culture Microtiter Plate 2 units DCFH-DA Probe (1000X) 1 x 100µl Free Radical Initiator 1 x 200mg Quercetin 1 x 250µl -
Research areas
Images
-
Cellular Antioxidant Activity of Quercetin in HeLa Cells.
60,000 HeLa cells were seeded and cultured in a 96-well plate until confluent. Cells were then pretreated with DCFH-DA and Quercetin for 60 minutes at 37ºC. Free Radical Initiator was then added to the cells to begin the assay, which was read every five minutes for 1 hour at 37ºC.
-
Dose-Response Curve of Quercetin Standard. -
Example Data for Calculation of AUC for Antioxidant Sample and Negative Control.
