Human Peroxiredoxin 1 ELISA Kit (PRX1) (ab185983)
Key features and details
- Sensitivity: 200 pg/ml
- Range: 0.27 ng/ml - 200 ng/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Peroxiredoxin 1 ELISA Kit (PRX1) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 4.15% Inter-assay Sample n Mean SD CV% Overall 3 7.3% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
200 pg/ml -
Range
0.27 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 82 70% - 97% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Peroxiredoxin 1 (PRX1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Peroxiredoxin 1 protein in human cell and tissue extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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Notes
Peroxiredoxins are a widely-expressed family of antioxidant enzymes involved in redox regulation and oxidative stress defense. Their ability to reduce hydrogen peroxide by utilizing thioredoxin as an electron donor is used by cells to regulate intracellular hydrogen peroxide levels. Additionally, the inactivation of peroxiredoxins by high levels of hydrogen peroxide and the ATP-dependent reactivation by sulfiredoxin indicates peroxiredoxins have a role in redox signaling.
Peroxiredoxin isoforms are grouped into two categories, 1-Cys and 2-Cys, based on the number of redox-active cysteine residues used in their catalytic mechanisms. The peroxiredoxin isoforms can also be characterized by their subcellular locations where PRX1, 2 and 6 are found in cytosol, PRX3 within mitochondria, and PRX4 is secreted and found in the ER.
Specifically, PRX1’s regeneration by reduction with thioredoxin but not glutathione signifies the isoform’s unique function as a regulator of hydrogen peroxide levels within cells.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X HRP Label 1 x 1ml 10X PRX1 Detector Antibody 1 x 1ml 10X Wash Buffer 1 x 40ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Detergent 1 x 1ml HRP Development Solution 1 x 12ml PRX1 96-Well Microplate 1 unit PRX1 Lyophilized Recombinant Protein 1 vial Stop Solution 1 x 12ml -
Research areas
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Cellular localization
Cytoplasm. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. -
Alternative names
- MSP23
- Natural killer cell enhancing factor A
- Natural killer enhancing factor A
see all -
Database links
- Entrez Gene: 5052 Human
- Omim: 176763 Human
- SwissProt: Q06830 Human
- Unigene: 180909 Human
Images
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Example of PRX1 protein standard curve.
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Recombinant Human PRX1, 2, 3, 4, and 6 were analyzed using the PRX1 Human ELISA Kit at 200 ng/mL each.The assay is specific to only PRX1 and does not recognize any of the other peroxiredoxin isoforms tested.