Anti-4 Hydroxynonenal antibody [12F7] (ab243070)
![Anti-4 Hydroxynonenal antibody [12F7] (ab243070)](https://www.abcam.com/ps/products/243/ab243070/Images/ab243070-332311-70.png "Anti-4 Hydroxynonenal antibody [12F7] (ab243070)")
Key features and details
- Mouse monoclonal [12F7] to 4 Hydroxynonenal
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-4 Hydroxynonenal antibody [12F7]
See all 4 Hydroxynonenal primary antibodies -
Description
Mouse monoclonal [12F7] to 4 Hydroxynonenal -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to 4 Hydroxynonenal conjugated to keyhole limpet haemocyanin.
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Positive control
- WB: 4 hydroxylnonenal-BSA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
12F7 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-4 Hydroxynonenal antibody [12F7] (ab243070)All lanes : Anti-4 Hydroxynonenal antibody [12F7] (ab243070) at 1/1000 dilution
Lane 1 : BSA at 0.5 µg
Lane 2 : 4 hydroxylnonenal-BSA at 0.5 µg
Lane 3 : 4 hydroxylnonenal-BSA at 2 µg
Lane 4 : 4-hydroxy-2-hexenal at 0.5 µg
Lane 5 : 4-hydroxy-2-hexenal at 2 µg
Secondary
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesPrimary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.