HORAC Assay Kit (ab242299)
Overview
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Product name
HORAC Assay Kit
See all Oxidative Stress kits -
Product overview
HORAC Assay Kit (ab242299) is a fast and reliable kit for the direct measurement of HORAC antioxidant capacity.
Each kit provides sufficient reagents to perform up to 192 assays, including blanks, antioxidant standards and unknown samples. The assay is designed for use in single plate microplate readers as well as readers with high-throughput capabilities.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 192 tests 48 tests 96-well Microtiter Plate 2 units 2 units Assay Diluent (2X) 1 x 50ml 1 x 10ml Fenton Reagent 1 x 5ml 1 x 1ml Gallic Acid Antioxidant Standard 1 x 1.5ml 1 x 1.5ml HORAC Fluorescein Probe (100X) 1 x 0.5ml 1 x 75µl Hydroxyl Radical Initiator (5X) 1 x 1ml 1 x 200µl -
Research areas
Images
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HORAC Activity Assay Standard Curve.
The figure demonstrates typical Assay results. One should use the data for reference only. This data should not be used to interpret or calculate actual sample results.
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Calculating resultsCalculate the area under the curve (AUC) for each sample and standard using the final assay values and the linear regression formula as shown in the protocol booklet.
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Calculating resultsCalculate the Net AUC by subtracting the Blank AUC from the AUC of each sample and standard.
