Recombinant human PRMT10 protein (Active) (ab271723)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 83% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PRMT10 protein (Active)
See all PRMT10 proteins and peptides -
Biological activity
Assay Description: 10 μl reaction mix containing assay buffer with S-adenosylmethionine, specific PRMT9 substrate, and PRMT9 (30-100 ng) added to the wells. Add antibody against methylated Arg residue and A-screen Acceptor beads. Incubate for 30 mins, then add GSH-conjugated Donor beads followed by Alpha Screening detection.
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Purity
>= 83 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
SNSRPRSRR DAGGGAGAAG RDELVSRSLQ SAEHCLGVQD FGTAYAHYLL VLSLAPELKH DVKETFQYTL FRWAEELDAL SRIQDLLGCY EQALELFPDD EVICNSMGEH LFRMGFRDEA AGYFHKAVKL NPDFSDAKEN FYRVANWLVE RWHFIMLNDT KRNTIYNAAI QKAVCLGSKS VLDIGAGTGI LSMFAKKAGA HSVYACELSK TMYELACDVV AANKMEAGIK LLHTKSLDIE IPKHIPERVS LVVTETVDAG LFGEGIVESL IHAWEHLLLQ PKTKGESANC EKYGKVIPAS AVIFGMAVEC AEIRRHHRVG IKDIAGIHLP TNVKFQSPAY SSVDTEETIE PYTTEKMSRV PGGYLALTEC FEIMTVDFNN LQELKSLATK KPDKIGIPVI KEGILDAIMV WFVLQLDDEH SLSTSPSEET CWEQAVYPVQ DLADYWIKPG DHVMMEVSCQ DCYLRIQSIS VLGLECEMDV AKSFTQNKDL LSLGNEAELC SALANLQTSK PDAVEQTCIL ESTEIALLNN IPYHEGFKMA MSKVLSSLTP EKLYQTMDTH CQNEMSSGTG QSNTVQNILE PFYVLDVSEG FSVLPVIAGT LGQVKPYSSV EKDQHRIALD LISEANHFPK ETLEFWLRHV EDESAMLQRP KSDKLWSIII LDVIEPSGLI QQEIMEKAAI SRCLLQSGGK IFPQYVLMFG LLVESQTLLE ENAVQGTERT LGLNIAPFIN QFQVPIRVFL DLSSLPCIPL SKPVELLRLD LMTPYLNTSN REVKVYVCKS GRLTAIPFWY HMYLDEEIRL DTSSEASHWK QAAVVLDNPI QVEMGEELVL SIQHHKSNVS ITVKQ -
Predicted molecular weight
97 kDa including tags -
Amino acids
2 to 845 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
C-terminal Strep-Tag
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Preparation and Storage
- ANM10_HUMAN
- Prmt10
- protein arginine methyltransferase 10 (putative)
- Putative protein arginine N-methyltransferase 10
Images
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Functional Studies - Recombinant human PRMT10 protein (Active) (ab271723)Specific activity of ab271723.
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SDS-PAGE - Recombinant human PRMT10 protein (Active) (ab271723)SDS-PAGE analysis of 2 µg ab271723.