Human Superoxide Dismutase 1 ELISA Kit (ab119520)
Key features and details
- Sensitivity: 0.04 ng/ml
- Range: 0.08 ng/ml - 5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Superoxide Dismutase 1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.1% Inter-assay Sample n Mean SD CV% Overall 5.8% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.04 ng/ml -
Range
0.08 ng/ml - 5 ng/ml -
Recovery
98 %
Sample specific recovery Sample type Average % Range Serum 98 89% - 108% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human Superoxide Dismutase 1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cu/Zn Superoxide Dismutase concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, fetal umbilical vein blood and urine.
Cu/Zn Superoxide Dismutase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a Cu/Zn Superoxide Dismutase HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the Cu/Zn Superoxide Dismutase amount of sample captured in plate.
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Notes
ab119520 Human Superoxide Dismutase 1 ELISA kit has been shown to detect both SOD1 and SOD3.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 2 units HRP-Conjugate anti-Human Cu/Zn Superoxide Dismutase monoclonal antibody 2 x 20µl Human Cu/Zn Superoxide Dismutase Standard (5 ng/mL) 2 x 500µl Microplate coated with anti Human Cu/Zn SOD monoclonal antibody (12 x 8 wells) 1 unit Phosphate Buffered Saline Concentrate (PBS) (20x) 1 x 5ml Stop Solution (1M Phosphoric acid) 1 x 15ml TMB Substrate Solution 1 x 15ml -
Research areas
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Cellular localization
Cytoplasmic. Note: The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. -
Alternative names
- ALS
- ALS1
- Amyotrophic Lateral Sclerosis 1 (Adult)
see all -
Database links
- Entrez Gene: 6647 Human
- Omim: 147450 Human
- SwissProt: P00441 Human
- Unigene: 443914 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab119520.
