Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIP30 antibody [EPR13179] (ab177961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13179] to TIP30
  • Suitable for: Flow Cyt, IP, IHC-P, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-TIP30 antibody [EPR13179]
    See all TIP30 primary antibodies

  • Description

    Rabbit monoclonal [EPR13179] to TIP30

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TIP30 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BUP3

  • Positive control

    • HeLa, A431, and fetal liver lysates; Human pancreas and placenta tissues; A431 cells; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-TIP30 antibody [EPR13179] (ab177961)
    Western blot - Anti-TIP30 antibody [EPR13179] (ab177961)
    All lanes : Anti-TIP30 antibody [EPR13179] (ab177961) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 27 kDa

  • Flow Cytometry - Anti-TIP30 antibody [EPR13179] (ab177961)
    Flow Cytometry - Anti-TIP30 antibody [EPR13179] (ab177961)

    Flow cytometric analysis of permeabilized HeLa cells labeling TIP30 using ab177961 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Immunocytochemistry/ Immunofluorescence - Anti-TIP30 antibody [EPR13179] (ab177961)
    Immunocytochemistry/ Immunofluorescence - Anti-TIP30 antibody [EPR13179] (ab177961)

    Immunofluorescence analysis of A431 cells labeling TIP30 using ab177961 at 1/50 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)

    Immunohistochemical analysis of Human placenta tissue labeling TIP30 using ab177961 at 1/50 dilution.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)

    Immunohistochemical analysis of Human pancreas tissue labeling TIP30 using ab177961 at 1/50 dilution.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-TIP30 antibody [EPR13179] (ab177961)
    Immunoprecipitation - Anti-TIP30 antibody [EPR13179] (ab177961)

    Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab177961 at 1/10 dilution to immunoprecipitate TIP30, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-TIP30 antibody [EPR13179] (ab177961)
    Anti-TIP30 antibody [EPR13179] (ab177961)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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