Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19814] to CSC1 - BSA and Azide free
  • Suitable for: WB, IP, ICC
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CSC1 antibody [EPR19814] - BSA and Azide free
    See all CSC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19814] to CSC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    Recombinant fragment within Mouse CSC1 aa 200-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q8CBX0

    Recombinant fragment within Mouse CSC1 aa 700 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q8CBX0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    ab251395 is the carrier-free version of ab203486 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251395 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as TMEM63C

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR19814

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
    Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
    All lanes : Anti-CSC1 antibody [EPR19814] (ab203486) at 1/1000 dilution

    Lane 1 : Mouse cerebral cortex tissue lysate
    Lane 2 : Mouse cerebellum tissue lysate
    Lane 3 : Rat cerebellum tissue lysate
    Lane 4 : Rat cerebral cortex tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa



    This data was developed using ab203486, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lanes 2-4: 10 seconds.

    To avoid the formation of CSC1 high molecule weight aggregates the lysates in sample buffer were not boiled prior to WB testing.

  • Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
    Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395) The Images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University, MARC.
    All lanes : Anti-CSC1 antibody [EPR19814] (ab203486) at 1/400 dilution

    Lane 1 : CSC1 KO (-/-) mouse brain lysate
    Lane 2 : CSC1 WT (+/+) mouse brain lysate
    Lane 3 : CSC1 heterozygous (+/-) mouse brain lysate

    Secondary
    All lanes : Goat Anti-Mouse IgG (H+L) HRP at 1/15000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa


    Exposure time: 30 seconds


    This data was developed using ab203486, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
    Immunocytochemistry - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
    This data was developed using ab203486, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HA-mCSC1 transfected HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling CSC1 with ab203486 at 1/150 dilution (green). Counterstained with Anti-HA antibody at 1/500 dilution (red). Positive staining on HA-mCSC1 transfected HEK-293T cells. The images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC. The nuclear counter stain is DAPI (blue).
  • Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
    Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
    This data was developed using ab203486, the same antibody clone in a different buffer formulation.CSC1 was immunoprecipitated from 0.35 mg of mouse cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse cerebellum lysate 10 μg (Input). Lane 2: ab203486 IP in mouse cerebellum lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203486 in mouse cerebellum lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds. Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.
  • Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
    Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
    This data was developed using ab203486, the same antibody clone in a different buffer formulation.CSC1 was immunoprecipitated from 0.35 mg of rat cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Rat cerebellum lysate 10 μg (Input). Lane 2: ab203486 IP in rat cerebellum lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203486 in rat cerebellum lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds. Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.
  • Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)
    Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)

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