Recombinant human UBE1 protein (Active) (ab269091)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human UBE1 protein (Active)
See all UBE1 proteins and peptides -
Biological activity
The specific activity of ab269091 was 110 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.
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Purity
> 95 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Molecular weight information
145 kDa by SDS-PAGE -
Tags
GST tag N-Terminus -
Additional sequence information
NM_003334
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Preparation and Storage
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Alternative names
- A1S9
- A1S9 protein
- A1S9T
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Relevance
Ubiquitinylation, the covalent attachment of ubiquitin to protein, regulates a number of cellular processes including responses to stress. This process is responsible for selective targeting of proteins for degradation. The first step in ubiquitinylation is the activation of ubiquitin by ubiquitin activating enzyme E1 which is a rate-limiting enzyme in the conjugation process. The ubiquitin activating enzyme exists as two isoforms: E1a (117 kDa), localized predominantly in the nucleus, and E1b (110 kDa), localized in the cytoplasm. The first 11 amino acids are considered to be essential for phosphorylation and exclusive nuclear localization of the enzyme. -
Cellular localization
Cytoplasmic and Nuclear
Images
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Functional Studies - Recombinant human UBE1 protein (ab269091)
The specific activity of ab269091 was 110 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.
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SDS-PAGE - Recombinant human UBE1 protein (ab269091)SDS-PAGE analysis of ab269091.