Anti-PDHB antibody [EPR11097(B)] (ab155996)
![Anti-PDHB antibody [EPR11097(B)] (ab155996)](https://www.abcam.com/ps/products/155/ab155996/Images/ab155996-163326-anti-pyruvate-dehydrogenase-e1-beta-subunit-antibody-epr11097b-western-blot.jpg "Anti-PDHB antibody [EPR11097(B)] (ab155996)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11097(B)] to PDHB
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-PDHB antibody [EPR11097(B)]
See all PDHB primary antibodies -
Description
Rabbit monoclonal [EPR11097(B)] to PDHB -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
Immunogen
corresponding to Human PDHB.
Database link: P11177 -
Positive control
- HepG2, HeLa, A375 and Human fetal heart lysates; Human colon tissue; Human thyroid gland carcinoma tissue; HeLa cells.
-
General notes
This product was previously labelled as Pyruvate Dehydrogenase E1 beta subunit
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11097(B) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PDHB antibody [EPR11097(B)] (ab155996)All lanes : Anti-PDHB antibody [EPR11097(B)] (ab155996) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A375 cell lysate
Lane 4 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 35 kDa
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)](https://www.abcam.com/ps/products/155/ab155996/Images/ab155996-168840-anti-pyruvate-dehydrogenase-e1-beta-subunit-antibody-epr11097b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)ab155996 showing +ve staining in Human normal pancreas.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)](https://www.abcam.com/ps/products/155/ab155996/Images/ab155996-168839-anti-pyruvate-dehydrogenase-e1-beta-subunit-antibody-epr11097b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)ab155996 showing +ve staining in Human ovarian carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)](https://www.abcam.com/ps/products/155/ab155996/Images/ab155996-168838-anti-pyruvate-dehydrogenase-e1-beta-subunit-antibody-epr11097b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)ab155996 showing +ve staining in Human normal kidney.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)](https://www.abcam.com/ps/products/155/ab155996/Images/ab155996-168837-anti-pyruvate-dehydrogenase-e1-beta-subunit-antibody-epr11097b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)ab155996 showing +ve staining in Human ovarian carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)](https://www.abcam.com/ps/products/155/ab155996/Images/ab155996-163325-anti-pyruvate-dehydrogenase-e1-beta-subunit-antibody-epr11097b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PDHB with ab155996 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)](https://www.abcam.com/ps/products/155/ab155996/Images/ab155996-163324-anti-pyruvate-dehydrogenase-e1-beta-subunit-antibody-epr11097b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling PDHB with ab155996 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-PDHB antibody [EPR11097(B)] (ab155996)](https://www.abcam.com/ps/products/155/ab155996/Images/ab155996-163323-anti-pyruvate-dehydrogenase-e1-beta-subunit-antibody-epr11097b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PDHB antibody [EPR11097(B)] (ab155996)Immunofluorescence analysis of HeLa cells labeling PDHB with ab155996 at 1/100 dilution.
-
![Anti-PDHB antibody [EPR11097(B)] (ab155996)](https://www.abcam.com/ps/products/155/ab155996/Images/ab155996-9-benefits-of-recombinant-antibodies.png)
Anti-PDHB antibody [EPR11097(B)] (ab155996)
