Anti-PDHB antibody [EPR11096(B)] (ab155953)
![Anti-PDHB antibody [EPR11096(B)] (ab155953)](https://www.abcam.com/ps/products/155/ab155953/Images/Pyruvate-Dehydrogenase-E1-beta-subunit-Primary-antibodies-ab155953-1.JPG "Anti-PDHB antibody [EPR11096(B)] (ab155953)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11096(B)] to PDHB
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-PDHB antibody [EPR11096(B)]
See all PDHB primary antibodies -
Description
Rabbit monoclonal [EPR11096(B)] to PDHB -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
corresponding to Human PDHB.
Database link: P11177 -
Positive control
- HepG2, HeLa, A375 and Human fetal heart lysates; Human colon and pancreas tissues; HepG2 and A375 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Pyruvate Dehydrogenase E1 beta subunit
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11096(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDHB antibody [EPR11096(B)] (ab155953)All lanes : Anti-PDHB antibody [EPR11096(B)] (ab155953) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : HeLa cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11096(B)] (ab155953)](https://www.abcam.com/ps/products/155/ab155953/Images/ab155953-9-YCAR11096B-PDHB-thyroid-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11096(B)] (ab155953)ab155953 showing +ve staining in Human thyroid gland carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11096(B)] (ab155953)](https://www.abcam.com/ps/products/155/ab155953/Images/ab155953-8-YCAR11096B-PDHB-ovarian-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11096(B)] (ab155953)ab155953 showing +ve staining in Human ovarian carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11096(B)] (ab155953)](https://www.abcam.com/ps/products/155/ab155953/Images/ab155953-7-YCAR11096B-PDHB-heart.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11096(B)] (ab155953)ab155953 showing +ve staining in Human heart.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11096(B)] (ab155953)](https://www.abcam.com/ps/products/155/ab155953/Images/ab155953-6-YCAR11096B-PDHB-gastric-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11096(B)] (ab155953)ab155953 showing +ve staining in Human gastric adenocarcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PDHB antibody [EPR11096(B)] (ab155953)](https://www.abcam.com/ps/products/155/ab155953/Images/Pyruvate-Dehydrogenase-E1-beta-subunit-Primary-antibodies-ab155953-2.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-PDHB antibody [EPR11096(B)] (ab155953)Immunofluorescent analysis of HepG2 cells, labeling PDHB with ab155953 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11096(B)] (ab155953)](https://www.abcam.com/ps/products/155/ab155953/Images/Pyruvate-Dehydrogenase-E1-beta-subunit-Primary-antibodies-ab155953-3.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11096(B)] (ab155953)Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling PDHB with ab155953 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11096(B)] (ab155953)](https://www.abcam.com/ps/products/155/ab155953/Images/Pyruvate-Dehydrogenase-E1-beta-subunit-Primary-antibodies-ab155953-4.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11096(B)] (ab155953)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, labeling PDHB with ab155953 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-PDHB antibody [EPR11096(B)] (ab155953)](https://www.abcam.com/ps/products/155/ab155953/Images/Pyruvate-Dehydrogenase-E1-beta-subunit-Primary-antibodies-ab155953-5.JPG)
Flow Cytometry - Anti-PDHB antibody [EPR11096(B)] (ab155953)Flow cytometric analysis of permeabilized A375 cells labeling PDHB with ab155953 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).
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![Anti-PDHB antibody [EPR11096(B)] (ab155953)](https://www.abcam.com/ps/products/155/ab155953/Images/ab155953-10-benefits-of-recombinant-antibodies.png)
Anti-PDHB antibody [EPR11096(B)] (ab155953)
