Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDHB antibody [17A5E2H8] (ab110331)

Key features and details

  • Mouse monoclonal [17A5E2H8] to PDHB
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PDHB antibody [17A5E2H8]
    See all PDHB primary antibodies

  • Description

    Mouse monoclonal [17A5E2H8] to PDHB

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Immunogen

    Full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human, Bovine, Rat and Mouse hearts; HepG2 whole cell lysate (ab7900). This antibody gave a positive result in IHC in the following FFPE tissue: Human normal duodenum.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [17A5E2H8] (ab110331)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [17A5E2H8] (ab110331)

    IHC image of PDHB staining in Human normal duodenum formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110331, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-PDHB antibody [17A5E2H8] (ab110331)
    Western blot - Anti-PDHB antibody [17A5E2H8] (ab110331)
    All lanes : Anti-PDHB antibody [17A5E2H8] (ab110331) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 10 µg/ml
    Lane 2 : Isolated mitochondria from Bovine heart at 4 µg/ml
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg/ml
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg/ml
    Lane 5 : HepG2 cell lysate at 20 µg/ml

    Predicted band size: 39 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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