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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family

Anti-Ras antibody (ab180772)

Price and availability

308 236 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Ras antibody (ab180772)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Ras
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Ras antibody
    See all Ras primary antibodies
  • Description

    Rabbit polyclonal to Ras
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 82% homology with HRAS and NRAS. Cross-reactivity with these proteins has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Ras aa 30-189.
    Sequence:

    D EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QDLARSYGIP FIETSAKTRQ RVEDAFYTLV REIRQYRLKK ISKEEKTPGC VKIKKCIIM


    Database link: P01116
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: A-549, HeLa and Raji cell lysates. Mouse spleen, lung and rat brain tissue extracts. ICC/IF: C6 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
    • Cancer
    • Tumor biomarkers
    • Oncoproteins

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody (ab180772)
    Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody (ab180772)

    Immunofluorescence staining of C6 cells stained for RAS with ab180772 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

  • Western blot - Anti-Ras antibody (ab180772)
    Western blot - Anti-Ras antibody (ab180772)
    All lanes : Anti-Ras antibody (ab180772) at 1/1000 dilution

    Lane 1 : A-549 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : Raji cell extract
    Lane 4 : Mouse spleen tissue extract
    Lane 5 : Mouse lung tissue extract
    Lane 6 : Rat brain tissue extract

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP Goat Anti-Rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 21 kDa


    Exposure time: 90 seconds


    Blocking buffer: 3% nonfat dry milk in TBST.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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