Human NRP2 ELISA kit (ab277470)
Key features and details
- Sensitivity: 8.1 ng/ml
- Range: 8.192 ng/ml - 2000 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human NRP2 ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.1 ng/ml -
Range
8.192 ng/ml - 2000 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 95.19 79% - 103% Plasma 81.76 68% - 106% Cell culture media 98.15 90% - 108% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human NRP2 ELISA Kit (ab277470) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NRP2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human NRP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human NRP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human NRP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human NRP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human NRP2 Antibody 2 vials Anti-Human NRP2 coated Microplate 1 unit Human NRP2 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF. -
Sequence similarities
Belongs to the neuropilin family.
Contains 2 CUB domains.
Contains 2 F5/8 type C domains.
Contains 1 MAM domain. -
Domain
The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- MGC126574
- Neuropilin 2
- Neuropilin-2
see all -
Database links
- Entrez Gene: 8828 Human
- Omim: 602070 Human
- SwissProt: O60462 Human
- Unigene: 471200 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.