Human Protein Z ELISA Kit (ab137989)
Key features and details
- Sensitivity: 0.9 ng/ml
- Range: 89 % - 112 %
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Protein Z ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.2% Inter-assay Sample n Mean SD CV% Overall 8.7% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
> 0.9 ng/ml -
Range
89 % - 112 % -
Recovery
96 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Protein Z Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Protein Z in plasma, serum, urine, milk, and cell culture supernatants.
A Protein Z specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Protein Z specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Protein Z captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Protein Z Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Protein Z Microplate (12 x 8 well strips) 1 unit Protein Z Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. -
Tissue specificity
Plasma. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Protein Z, vitamin K dependent plasma glycoprotein
- PROZ
- PROZ_HUMAN
see all -
Database links
- Entrez Gene: 8858 Human
- Omim: 176895 Human
- SwissProt: P22891 Human
- Unigene: 1011 Human
Images
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ELISA: Protein Z Human ELISA Kit (ab137989)
Standard curve with background signal subtracted (duplicates; +/- SD).
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ELISA: Protein Z Human ELISA Kit (ab137989)Protein Z measured in biological fluids (duplicates +/- SD). Human plasma and serum were tested at a dilution factor range of 50-1350, other fluids 1-10.
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Typical Standard CurveRepresentative Standard Curve using ab137989.