Astana Biomed Group, an authorized Abcam distributor in Central Asia

Porcine PDGF-BB ELISA (ab218297)

Key features and details

  • Sensitivity: 10 pg/ml
  • Range: 10 pg/ml - 2500 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human, Pig

Overview

  • Product name

    Porcine PDGF-BB ELISA
    See all PDGF B kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1
    Inter-assay
    Sample n Mean SD CV%
    1

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    10 pg/ml

  • Range

    10 pg/ml - 2500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 120.5 108% - 130%
    Serum 108.6 87% - 123%
    Plasma 112.3 98% - 115%

  • Assay time

    4h 45m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human, Pig

  • Product overview

    The Porcine PDGF-BB ELISA kit (ab218297) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of porcine PDGF-BB in serum, plasma and cell culture supernatants. 


    This assay employs an antibody specific for porcine PDGF-BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF-BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine PDGF-BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Porcine PDGF-BB ELISA kit (ab218297) Standard curve.
    Porcine PDGF-BB ELISA kit (ab218297) Standard curve.

    Porcine PDGF-BB ELISA kit (ab218297) Standard curve.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.