Porcine PDGF-BB ELISA (ab218297)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 10 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Pig
Overview
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Product name
Porcine PDGF-BB ELISA
See all PDGF B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 Inter-assay Sample n Mean SD CV% 1 -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10 pg/ml -
Range
10 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 120.5 108% - 130% Serum 108.6 87% - 123% Plasma 112.3 98% - 115% -
Assay time
4h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human, Pig -
Product overview
The Porcine PDGF-BB ELISA kit (ab218297) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of porcine PDGF-BB in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for porcine PDGF-BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF-BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine PDGF-BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 800X Streptavidin-Conjugated HRP 1 x 200µl Anti-Human PDGF-BB coated Microplate (12 x 8 wells) 1 x 96 tests Biotinylated (PDGF-BB) Detection Antibody 2 vials Lyophilized (PDGF-BB) Standard 2 vials Stop Solution 1 x 8ml TMB Substrate 1 x 12ml -
Research areas
- Information by UniProt
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Alternative names
- Becaplermin
- c sis
- FLJ12858
see all -
Database links
- Entrez Gene: 5155 Human
- Omim: 190040 Human
- SwissProt: P01127 Human
- SwissProt: P20034 Pig
- Unigene: 1976 Human
Images
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Porcine PDGF-BB ELISA kit (ab218297) Standard curve.
Porcine PDGF-BB ELISA kit (ab218297) Standard curve.
