Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP3976] to NuMA
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-NuMA antibody [EP3976]
See all NuMA primary antibodies
Description

Rabbit monoclonal [EP3976] to NuMA
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- K562, HeLa, MCF7, Raji, SW480, CACO2, and MOLT4 cell lysates; Human breast carcinoma, normal breast and colonic adenocarcinoma tissues; HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP3976
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-NuMA antibody [EP3976] (ab109262)

ab109262, at 1/100 dilution, staining NuMA in HeLa cells by Immunofluorescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] (ab109262)

ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human colonic adenocarcinoma by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] (ab109262)

ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast carcinoma by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] (ab109262)

ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-NuMA antibody [EP3976] (ab109262)

All lanes : Anti-NuMA antibody [EP3976] (ab109262) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Raji cell lysate
Lane 5 : SW480 cell lysate
Lane 6 : CACO2 cell lysate
Lane 7 : MOLT4 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 238 kDa
Anti-NuMA antibody [EP3976] (ab109262)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com