Anti-NuMA antibody (ab241614)
Key features and details
- Rabbit polyclonal to NuMA
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NuMA antibody
See all NuMA primary antibodies -
Description
Rabbit polyclonal to NuMA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NuMA aa 900-950. The exact sequence is proprietary. (NP_006176.2).
Database link: Q14980 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa cell lysate. ICC/IF: Human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to NuMA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (ab241614)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for NuMA using ab241614 at 1/400 dilution in immunohistochemical analysis. Secondary antibody used was a red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed antibody DyLight®594 conjugated antibody used at a 1/100 dilution.
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Immunoprecipitation - Anti-NuMA antibody (ab241614)NuMA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241614 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241614 at 0.1 µg/ml.
Lane 1: ab241614 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 seconds.
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Western blot - Anti-NuMA antibody (ab241614)All lanes : Anti-NuMA antibody (ab241614) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 238 kDa
Exposure time: 3 seconds
