Anti-NuMA antibody (ab84680)
Key features and details
- Rabbit polyclonal to NuMA
- Suitable for: IHC-P
- Reacts with: Human, Common marmoset
- Isotype: IgG
Overview
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Product name
Anti-NuMA antibody
See all NuMA primary antibodies -
Description
Rabbit polyclonal to NuMA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide corresponding to a region between residue 900 and 950 of Human NuMA (NP_006176.2).
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Positive control
- Human Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Lung Adenocarcinoma, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Skin Melanoma, Stomach Adenocarcinoma and Testicular Seminoma.
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General notes
Concentration is optimized for IHC and not determined
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (ab84680) This image is courtesy of an anonymous Abreview
ab84680 staining NuMA in Marmoset (common) testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Buoin's fixative, permeabilized in Triton X-100 (1/100 in PBS) and blocked with 25% serum for 30 minutes; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/100 in PBS + 0.5% BSA) for 17 hours at 4°C. A Biotin-conjugated Chicken anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (ab84680)ab84680, at 1/100 dilution, staining NuMA in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (ab84680)ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using DAB staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (ab84680)ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry using DAB staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (ab84680)ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human ovarian tumor by Immunohistochemistry using DAB staining.
