Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Villin antibody [rVIL1/1325rVIL1/1325] - BSA and Azide free (ab268252)

Key features and details

Mouse monoclonal [rVIL1/1325] to Villin - BSA and Azide free
Suitable for: Protein Array, IHC-P
Reacts with: Human
Isotype: IgG1

Product name

Anti-Villin antibody [rVIL1/1325] - BSA and Azide free
See all Villin primary antibodies
Description

Mouse monoclonal [rVIL1/1325] to Villin - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: Protein Array, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

This product was produced with the following immunogens:
Recombinant fragment within Human Villin aa 179-311. The exact sequence is proprietary.
Database link: P09327

Recombinant fragment within Human Villin aa 179-311. The exact sequence is proprietary.
Database link: P09327
Positive control
- IHC-P: Human small intestinal carcinoma tissue.
General notes

ab268252 is a carrier free version of ab268129.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from bioreactor concentrate.
Clonality

Monoclonal
Clone number

rVIL1/1325
Isotype

IgG1
Light chain type

kappa
Research areas

Protein Array - Anti-Villin antibody [rVIL1/1325] - BSA and Azide free (ab268252)

Protein Array containing more than 19,000 full-length human proteins using ab268129.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab268129).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [rVIL1/1325] - BSA and Azide free (ab268252)

Formalin-fixed, paraffin-embedded human small intestinal carcinoma tissue stained for Villin using ab268129 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab268129).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [rVIL1/1325] - BSA and Azide free (ab268252)

Formalin-fixed, paraffin-embedded human small intestinal carcinoma tissue stained for Villin using ab268129 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab268129).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [rVIL1/1325] - BSA and Azide free (ab268252)

Formalin-fixed, paraffin-embedded human small intestinal carcinoma tissue stained for Villin using ab268129 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268129)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [rVIL1/1325] - BSA and Azide free (ab268252)

Formalin-fixed, paraffin-embedded human small intestinal carcinoma tissue stained for Villin using ab268129 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268129)
Protein Array - Anti-Villin antibody [rVIL1/1325] - BSA and Azide free (ab268252)

Protein Array containing more than 19,000 full-length human proteins using ab268129.

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268129)
Anti-Villin antibody [rVIL1/1325rVIL1/1325] - BSA and Azide free (ab268252)

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