Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin beta 4 antibody [EPR17517] (ab182120)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17517] to Integrin beta 4
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Integrin beta 4 antibody [EPR17517]
    See all Integrin beta 4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17517] to Integrin beta 4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse spleen, kidney, pancreas, ovary and skin lysates; Human skin and pancreas lysates; BxPC-3 and SW480 whole cell lysates. IHC-P: Human colon, Human cervical carcinoma and mouse pancreas tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17517

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)

    Lane 1: Wild-type HAP1 whole cell lysate (40 µg)              
    Lane 2: ITGB4 knockout  HAP1 whole cell lysate (40 µg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab182120 observed at 240 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab182120 was shown to specifically recognize ITGB4 in wild-type HAP1 cells alond with additional cross-reactive bands. No band was observed when ITGB4 knockout samples were used. Wild-type and ITGB4 knockout samples were subjected to SDS-PAGE.  Ab182120 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/30,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling Integrin beta 4 with ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on Human cervical carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Integrin beta 4 with ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cell junction staining on mouse pancreatic acinus is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin beta 4 with ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on Human colon epithelium is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    All lanes : Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 202 kDa
    Observed band size: 210 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    All lanes : Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution

    Lane 1 : Mouse pancreas lysate
    Lane 2 : Mouse ovary lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 202 kDa
    Observed band size: 210 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/20000 dilution + Mouse skin lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 202 kDa
    Observed band size: 210 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/20000 dilution + Human skin lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 202 kDa
    Observed band size: 210 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution + Human pancreas lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 202 kDa
    Observed band size: 210 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution + BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 202 kDa
    Observed band size: 210 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/5000 dilution + SW480 (Human colon adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 202 kDa
    Observed band size: 210 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
    Anti-Integrin beta 4 antibody [EPR17517] (ab182120)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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