Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
![Anti-Integrin beta 4 antibody [EPR8559] (ab133682)](https://www.abcam.com/ps/products/133/ab133682/Images/ab133682-147670-anti-integrin-beta-4-antibody-epr8559-western-blot.jpg "Anti-Integrin beta 4 antibody [EPR8559] (ab133682)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8559] to Integrin beta 4
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Integrin beta 4 antibody [EPR8559]
See all Integrin beta 4 primary antibodies -
Description
Rabbit monoclonal [EPR8559] to Integrin beta 4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human Integrin beta 4 aa 1750-1850 (C terminal). The exact sequence is proprietary.
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Positive control
- BxPC-3 and SW480 cell lysates; BxPC-3 cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8559 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)All lanes : Anti-Integrin beta 4 antibody [EPR8559] (ab133682) at 1/1000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : SW480 cell lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 202 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)](https://www.abcam.com/ps/products/133/ab133682/Images/ab133682-226360-anti-integrin-beta-4-antibody-epr8559-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682) This image is courtesy of an Abreview submitted by Qingsu Chengab133682 staining Integrin beta 4 in human breast epithelail cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)](https://www.abcam.com/ps/products/133/ab133682/Images/ab133682-147669-anti-integrin-beta-4-antibody-epr8559-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)Immunofluorescent analysis of BxPC-3 cells labelling Integrin beta 4 with ab133682 at 1/250 dilution. -
![OI-RD Scanning - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)](https://www.abcam.com/ps/products/133/ab133682/Images/ab133682-147668-anti-integrin-beta-4-antibody-epr8559.jpg)
OI-RD Scanning - Anti-Integrin beta 4 antibody [EPR8559] (ab133682) -
![Anti-Integrin beta 4 antibody [EPR8559] (ab133682)](https://www.abcam.com/ps/products/133/ab133682/Images/ab133682-5-benefits-of-recombinant-antibodies.png)
Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
