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Anti-Integrin beta 4 antibody [M126] (ab29042)

Key features and details

  • Mouse monoclonal [M126] to Integrin beta 4
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Integrin beta 4 antibody [M126]
    See all Integrin beta 4 primary antibodies

  • Description

    Mouse monoclonal [M126] to Integrin beta 4

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    recombinant protein containing amino acid residues in the cytoplasmic region of human integrin beta 4.

  • Positive control

    • Flow Cyt (intra):A431 cell lysate. WB: 804G cells

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    protein A chromatography.

  • Clonality

    Monoclonal

  • Clone number

    M126

  • Isotype

    IgG1

  • Research areas

Images

  • Flow Cytometry - Anti-Integrin beta 4 antibody [M126] (ab29042)
    Flow Cytometry - Anti-Integrin beta 4 antibody [M126] (ab29042)

    Overlay histogram showing A431 cells stained with ab29042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29042, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Western blot - Anti-Integrin beta 4 antibody [M126] (ab29042)
    Western blot - Anti-Integrin beta 4 antibody [M126] (ab29042)
    All lanes : Anti-Integrin beta 4 antibody [M126] (ab29042)

    Lane 1 : A431 (25 µg/lane) serum starved overnight
    Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min

    Predicted band size: 202 kDa

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