Anti-Integrin beta 4 antibody [M126] (ab29042)
![Anti-Integrin beta 4 antibody [M126] (ab29042)](https://www.abcam.com/ps/products/29/ab29042/Images/ab29042-195222-anti-integrin-beta-4-antibody-m126-flow-cytometry.jpg "Anti-Integrin beta 4 antibody [M126] (ab29042)")
Key features and details
- Mouse monoclonal [M126] to Integrin beta 4
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Integrin beta 4 antibody [M126]
See all Integrin beta 4 primary antibodies -
Description
Mouse monoclonal [M126] to Integrin beta 4 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
recombinant protein containing amino acid residues in the cytoplasmic region of human integrin beta 4.
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Positive control
- Flow Cyt (intra):A431 cell lysate. WB: 804G cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
protein A chromatography. -
Clonality
Monoclonal -
Clone number
M126 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-Integrin beta 4 antibody [M126] (ab29042)
Overlay histogram showing A431 cells stained with ab29042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29042, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Western blot - Anti-Integrin beta 4 antibody [M126] (ab29042)](https://www.abcam.com/ps/products/29/ab29042/Images/ab29042-40190-anti-integrin-beta-4-antibody-m126-western-blot.jpg)
Western blot - Anti-Integrin beta 4 antibody [M126] (ab29042)All lanes : Anti-Integrin beta 4 antibody [M126] (ab29042)
Lane 1 : A431 (25 µg/lane) serum starved overnight
Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min
Predicted band size: 202 kDa
