Anti-Integrin beta 4 (phospho Y1526) antibody (ab29044)
Key features and details
- Rabbit polyclonal to Integrin beta 4 (phospho Y1526)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Integrin beta 4 (phospho Y1526) antibody
See all Integrin beta 4 primary antibodies -
Description
Rabbit polyclonal to Integrin beta 4 (phospho Y1526) -
Host species
Rabbit -
Specificity
The immunogen sequence used is found in all three integrin beta4 isoforms and is highly conserved in rat and mouse integrin beta4. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide conjugated to KLH, corresponding to amino acid residues around tyrosine 1526 of human integrin-beta4.
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Positive control
- A431 and src-transformed Hct116 cells treated with pervanadate.
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General notes
The cytoplasmic domain of the integrin-ß4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly, this site is critical for integrin a6ß4 increases in carcinoma invasion.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to dephosphorylated integrin beta4 (Tyr-1526) peptide before affinity purification using phospho-integrin beta-4(Tyr-1526) peptide (without carrier). -
Primary antibody notes
The cytoplasmic domain of the integrin-ß4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly, this site is critical for integrin a6ß4 increases in carcinoma invasion. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Integrin beta 4 (phospho Y1526) antibody (ab29044)All lanes : Anti-Integrin beta 4 (phospho Y1526) antibody (ab29044)
Lane 1 : A431 cell lysate, starved
Lane 2 : A431 cell lysate, treated with pervanadate for 30 minThe top band in each lane is 200 kDa.
