Anti-Kindlin 2 antibody (ab74030)
Key features and details
- Rabbit polyclonal to Kindlin 2
- Suitable for: IP, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Kindlin 2 antibody
See all Kindlin 2 primary antibodies -
Description
Rabbit polyclonal to Kindlin 2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human Kindlin 2 aa 450-550 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab74029) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IP Use a concentration of 5 µg/ml.WB (3) Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).ICC/IF (2) Use at an assay dependent concentration.Notes IP
Use a concentration of 5 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).ICC/IF
Use at an assay dependent concentration.Target
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Function
Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. -
Tissue specificity
Ubiquitous. Found in numerous tumor tissues. -
Sequence similarities
Belongs to the kindlin family.
Contains 1 FERM domain.
Contains 1 PH domain. -
Domain
The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain. -
Cellular localization
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Within actin stress fibers at cell-ECM focal adhesion sites. - Information by UniProt
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Database links
- Entrez Gene: 616981 Cow
- Entrez Gene: 10979 Human
- Entrez Gene: 218952 Mouse
- Entrez Gene: 289992 Rat
- Omim: 607746 Human
- SwissProt: Q96AC1 Human
- SwissProt: Q8CIB5 Mouse
- Unigene: 509343 Human
see all -
Alternative names
- FLJ34213 antibody
- FLJ44462 antibody
- UNC112 antibody
see all
Images
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Western blot - Anti-Kindlin 2 antibody (ab74030)All lanes : Anti-Kindlin 2 antibody (ab74030) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FERMT2 knockout HeLa cell lysate
Lane 3 : Saos-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDaLanes 1-3: Merged signal (red and green). Green - ab74030 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.
ab74030 Anti-Kindlin 2 antibody was shown to specifically react with Kindlin 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265247 (knockout cell lysate ab257436) was used. Wild-type and Kindlin 2 knockout samples were subjected to SDS-PAGE. ab74030 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Kindlin 2 antibody (ab74030)All lanes : Anti-Kindlin 2 antibody (ab74030) at 1 µg/ml
Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lane 2 : SAOS-2 (sarcoma osteogenic) Whole Cell lysate
Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Exposure time: 8 minutes -
Immunoprecipitation - Anti-Kindlin 2 antibody (ab74030)Kindlin 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Kindlin 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab74030.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 78kDa; Kindlin 2 -
Western blot - Anti-Kindlin 2 antibody (ab74030)This image is courtesy of an abreview submitted by Ana Kasirer-Friede,UCSD , United StatesThe kindlin 2 antibody (ab74030) was tested in lysate obtained from CHO cells either expressing endogenous levels of kindlin 2 (lane1)and transfected CHO cells in which flag-tagged kindlin2 was overexpressed (lane 2). A specific band was at the expected molecular weight of approximately 72 kDa (+/- Flag-tag). ß-actin was used to show loading in the two lanes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab74030 has been referenced in 13 publications.
- Xia W et al. Alzheimer's risk factor FERMT2 promotes the progression of colorectal carcinoma via Wnt/β-catenin signaling pathway and contributes to the negative correlation between Alzheimer and cancer. PLoS One 17:e0278774 (2022). PubMed: 36480537
- Boscher E et al. MicroRNA-138 Overexpression Alters Aß42 Levels and Behavior in Wildtype Mice. Front Neurosci 14:591138 (2020). PubMed: 33519353
- Boscher E et al. Copy Number Variants in miR-138 as a Potential Risk Factor for Early-Onset Alzheimer's Disease. J Alzheimers Dis 68:1243-1255 (2019). PubMed: 30909216
- Wei CY et al. Elevated kindlin-2 promotes tumour progression and angiogenesis through the mTOR/VEGFA pathway in melanoma. Aging (Albany NY) 11:6273-6285 (2019). PubMed: 31427543
- Karras P et al. p62/SQSTM1 Fuels Melanoma Progression by Opposing mRNA Decay of a Selective Set of Pro-metastatic Factors. Cancer Cell 35:46-63.e10 (2019). PubMed: 30581152
Images
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Western blot - Anti-Kindlin 2 antibody (ab74030)All lanes : Anti-Kindlin 2 antibody (ab74030) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FERMT2 knockout HeLa cell lysate
Lane 3 : Saos-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDaLanes 1-3: Merged signal (red and green). Green - ab74030 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.
ab74030 Anti-Kindlin 2 antibody was shown to specifically react with Kindlin 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265247 (knockout cell lysate ab257436) was used. Wild-type and Kindlin 2 knockout samples were subjected to SDS-PAGE. ab74030 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-Kindlin 2 antibody (ab74030)All lanes : Anti-Kindlin 2 antibody (ab74030) at 1 µg/ml
Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lane 2 : SAOS-2 (sarcoma osteogenic) Whole Cell lysate
Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Exposure time: 8 minutes
-
Immunoprecipitation - Anti-Kindlin 2 antibody (ab74030)Kindlin 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Kindlin 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab74030.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 78kDa; Kindlin 2 -
Western blot - Anti-Kindlin 2 antibody (ab74030) This image is courtesy of an abreview submitted by Ana Kasirer-Friede,UCSD , United StatesThe kindlin 2 antibody (ab74030) was tested in lysate obtained from CHO cells either expressing endogenous levels of kindlin 2 (lane1)and transfected CHO cells in which flag-tagged kindlin2 was overexpressed (lane 2). A specific band was at the expected molecular weight of approximately 72 kDa (+/- Flag-tag). ß-actin was used to show loading in the two lanes.
