Anti-Villin antibody (ab233155)
Key features and details
- Rabbit polyclonal to Villin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Villin antibody
See all Villin primary antibodies -
Description
Rabbit polyclonal to Villin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, Pig -
Immunogen
Recombinant fragment corresponding to Human Villin aa 1-350. Expressed in E. coli.
Database link: P09327 -
Positive control
- IHC-P: Human prostate, prostate cancer, stomach cancer, pancreatic cancer and breast cancer tissue. WB: Recombinant human Villin protein. Mouse kidney and pig liver tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Villin antibody (ab233155)All lanes : Anti-Villin antibody (ab233155) at 3 µg/ml
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Pig liver tissue lysate
Secondary
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 93 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab233155)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Villin with ab233155 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab233155)Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for Villin with ab233155 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab233155)Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for Villin with ab233155 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab233155)Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Villin with ab233155 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab233155)Formalin-fixed, paraffin-embedded human prostate tissue stained for Villin with ab233155 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Western blot - Anti-Villin antibody (ab233155)Anti-Villin antibody (ab233155) at 3 µg/ml + Recombinant human Villin protein
Predicted band size: 93 kDa
